Naše certifikace

Absolvovali jsme následující kurzy:


Course in Practical DNA Microarray Analysis (University of Heidelberg, 2007)
  • Quality control, normalization and design
  • Exercises: Introduction to R, cDNA data, affy data
  • Exploratory analysis
  • ifferential gene expression
  • Clustering
  • Annotation
  • Exercises: Differential gene expression, Clustering
  • Molecular Diagnosis
  • Classification with PAM and Random Forest
  • Model Assessment and Selection
  • Exercises: Molecular Diagnosis
  • Pathways
  • Computational Inference of Cellular Networks
  • Group testing: global tests, holistic approaches
  • Scoring Gene Ontology terms
  • Exercises: Group testing
Real-time PCR and biostatistics (TATAA Biocenter, Prague, 2010) - with practical part
  • Nucleic acid isolation and purification
  • Reverse transcription
  • Real time PCR quantification
  • Primer design
  • Validation of assays
  • Absolute quantification
  • Relative quantification
  • Identifying stable reference genes
  • Normalization
  • Experimental design
  • Basic principles of statistics
  • MIQE guidelines
  • HRM genotyping and methylation
  • HRM assay design
Primer and probe design and optimization for high-resolution melting analysis: design of primers for scanning HRMA and design of primers and unlabeled probes for SNP genotyping ( 07/2011, Idaho Inc., Salt Lake City, and KRD workshop, 10/2012, Prague, conducted by chief Idaho designers)
  • osobní návštěvy školitelů společnosti Idaho Inc.
BGI International Bioinformatics Workshop - analysis of NGS data (Shenzhen, China, 2012)
  • Applications of Sequencing in Human Disease Research 
  • From NGS-Genomics to MS-based Proteomics - Applications and Development in BGI
  • Genetic variation and marker discovery using Solexa sequencing technology
  • Small RNA research 
  • Transcriptome analysis 
  • Methods and software for de novo assembly 
  • Single Cell Omics 
  • Epigenomics by NGS


Primer and probe design for genotyping by HRMA and expression analysis by real-time quantitative PCR (KRD s.r.o., 2011-2012, Prague)

  • pravidelné interní firemní worskhopy o designu primerů a prób pro účely měření genové exprese pomocí real-time kvantitativní PCR a genotypování metodou vysokorozlišovací analýzy křivek tání